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. 2003 Feb;84(2):1031–1037. doi: 10.1016/S0006-3495(03)74919-4

FIGURE 3.

FIGURE 3

(A) Using a set of diaphragms, two regions of the membrane (large circle) were selected that were exposed to the UV flash or used for fluorescence measurements. (B) Different kinetics of flash triggered fluorescence changes at a distance s = 70 μm between both regions in the case of 1 mM caged H+ (blue) or 1 mM caged fluorescein (green) corresponding, respectively, to two-dimensional surface diffusion (Ds = 5.8 × 10−5 cm2/s) and three-dimensional bulk diffusion (Db = 2.8 × 10−6 cm2/s). In the former case fluorescence was collected from fluorescein labeled lipids (5%) incorporated into the membrane. Bulk solution: 100 mM NaCl, 0.1 (for H+) or 1 mM (for fluorescein) 1 mM CAPSO. The fit of the Monte Carlo simulations to the experimental data is shown by red triangles.