TABLE 1.

Interresidue FRET distances used to construct the cTnC/cTnI 129-160 models^*

Residues	FRET distance (Å)	Half-width (Å)	Model§ distance (Å)
cTnC 35 - cTnI 131	30.2	2.9	30.8
cTnC 35 - cTnI 146	27.0	4.1	28.4
cTnC 35 - cTnI 152	24.5	4.2	18.9
cTnC 35 - cTnI 160	26.4	2.7	27.0
cTnC 51 - cTnI 131	21.2	4.8	18.6
cTnC 51 - cTnI 146	30.5	6.4	17.8
cTnC 51 - cTnI 152	20.8	6.5	15.9
cTnC 51 - cTnI 160	23.7	5.8	22.8
cTnC 84 - cTnI 131	24.3	6.9	31.8
cTnC 84 - cTnI 146	23.5	4.0	25.3
cTnC 84 - cTnI 152	21.4	5.2	21.1
cTnC 84 - cTnI 160	24.7	4.4	27.9
cTnC 89 - cTnI 131	26.5	5.2	27.7
cTnC 89 - cTnI 146	24.3	4.3	20.2
cTnC 89 - cTnI 152	23.0	2.5	21.2
cTnC 89 - cTnI 160	24.8	5.2	25.1
cTnC 94 - cTnI 131	26.0	3.0	27.7
cTnC 94 - cTnI 146	24.3	4.1	20.2
cTnC 94 - cTnI 152	25.6	3.0	26.6
cTnC 94 - cTnI 160	28.7	7.0	28.2
cTnC 127 - cTnI 131	37.0	2.8	38.8
cTnC 127 - cTnI 146	28.4	3.7	37.2
cTnC 127 - cTnI 152	33.0	3.1	37.9
cTnC 127 - cTnI 160	37.1	5.0	44.0
cTnC 159 - cTnI 131	26.6	3.5	26.1
cTnC 159 - cTnI 146	25.5	3.8	22.2
cTnC 159 - cTnI 152	26.4	4.8	22.9
cTnC 159 - cTnI 160	29.7	5.0	28.6
cTnI 129 - cTnI 152†	28.4	6.9	20.9
cTnC 35 - cTnC 111‡	46.7	6.6

^*These are interresidue distances between cTnC and cTnI in which the cTnC regulatory N-domain is saturated with Ca²⁺. The distance is the mean distance of the distribution of the distances, and the half-width is the full width at half-maximum height of the distribution.

^†taken from Dong et al., 2001.

^‡taken from Dong et al., 2000.

^§inter-C-α distance measured from one structure of homology model.