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(Top) Absorption spectrum of wild-type LOV1 (solid line) and the long-lived intermediate LOV1-390 (dotted line) extracted by target factor analysis from the data shown below. (Bottom) Sequence of absorption spectra showing the relaxation of wild-type LOV1 from the photostationary state to the dark form after white-light irradiation. The inset shows the temporal behavior of the absorbance at 475 nm.
