FIGURE 2.

pH dependence of the decay time of the wild-type long-lived intermediate LOV1-390 at different sodium chloride concentrations (100 mM phosphate buffer). Time constants were derived from the recovery of absorbance at 475 nm with a maximum deviation of 3% from the average value. The lines are fitted curves corresponding to a general mechanistic model, which yields a pKa between 5 and 6 of the involved acid-base pair.