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Relative changes in absorbance of wild-type LOV1 at 475 nm dependent on the relative intensity of irradiation. The solid line is a fitted curve according to Eq. 4 reaching the limiting value of 0.84 at infinite light intensity. The dashed line illustrates the limit of 0.94 expected in the absence of a photoinduced back reaction from LOV1-390 to the dark form. This value results from the absorption of LOV1-390 at 475 nm according to Fig. 1.
