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. 2003 Feb;84(2):1208–1217. doi: 10.1016/S0006-3495(03)74935-2

FIGURE 2.

FIGURE 2

FIGURE 2

(A) Intermediate spectra of NpSRII obtained at 37°C with 2H2O. Spectra are labeled with the decay half-times of the intermediates: (a) 9.4 μs; (b) 0.645 ms; (c) 21.9 ms; (d) 183 ms. Spectral resolution, 8 cm−1. (B) Amplitude spectra of NpSRII obtained at 37°C with 2H2O from the time-resolved infrared spectra by global fitting to a sum of exponentials. The spectra are labeled with the respective half-times of the exponentials. Spectral resolution, 8 cm−1. Method used to derive the intermediate spectra (A) from the amplitude spectra (B) is described in Materials and Methods.