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Plots of ACS values versus the secondary structure contents estimated from the three-dimensional structures using the program PROMOTIF. (a) and (b) show the correlation of 1HN and 15N ACS values, respectively, to the percentage of β-sheet structures, whereas (c) and (d) correlate the same ACS values with respect to the percentage of helical structure (sum of α and 310 helices). Continuous lines represent the linear regression analysis results.
