FIGURE 5.

SNAP-23 associated proteins in pancreatic acinar cells. Total pancreatic homogenate was immunoprecipitated using the SNAP-23 specific antibody. The precipitated material was resolved using 12.5% SDS-PAGE, electrotransferred to nitrocellulose membrane, and then probed using antibodies to a number of proteins. Association of SNAP-23 with syntaxin 2; with cytoskeletal proteins actin, α-fodrin, and vimentin; and with calcium channels β3 and α1c, together with the SNARE regulatory protein NSF, is demonstrated (arrowheads). Lanes showing more than one arrowhead suggest presence of isomers or possible proteolytic degradation of the specific protein.