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. 2003 Feb;84(2):1399–1409. doi: 10.1016/S0006-3495(03)74954-6

FIGURE 2.

FIGURE 2

Effect of dephosphorylation/phosphorylation by PP2A/PKA-cs treatment on the activation of GIRK1/GIRK4 channels by Gβ1/γ2. (A, left) Current recorded from a multichannel i.o. patch at −80 mV (N = 3). 1 mM ATP and 134 mU/ml PP2A were present in the bath solution. Channel openings are represented by downward deflections of the current trace at −80 mV. First, currents were recorded in the cell attached (c.a.) configuration. The arrows indicate formation of an isolated (i.o.) patch, addition of 10 nM Gβ1/γ2, and later 100 nM Gβ1/γ2 to the bathing solution. Asterisks signify time spans when the membrane potential was switched to +80 mV to test for inward rectification of channel openings. (A, right) Same experimental paradigm as for current trace shown in the left panel, but 20 mU/ml PKA-cs was present in the bath solution instead of PP2A (N = 2). (B) P(o)*N, calculated for 10-s intervals, versus time, shown for the entire current traces visible in A.