TABLE 1.

Single channel open state parameters in inside-out patches

	Basal		10 nM Gβ1/γ2		100 nM Gβ1/γ2
	PKA-cs	PP2A	PKA-cs	PP2A	PKA-cs	PP2A
τo1 (ms)	0.22 ± 0.06	0.34 ± 0.01	0.66 ± 0.15	0.36 ± 0.08	0.73 ± 0.10	0.41 ± 0.03
τo2 (ms)	2.03 ± 0.51	1.67 ± 0.29	1.89 ± 0.14	1.86 ± 0.25	1.92 ± 0.14	2.29 ± 0.58
ao1	0.83** ± 0.12	0.20 ± 0.11	0.61 ± 0.14	0.67† ± 0.10	0.72 ± 0.10	0.69 ± 0.08
ao2	0.17 ± 0.12	0.80 ± 0.11	0.39 ± 0.14	0.33 ± 0.10	0.28 ± 0.10	0.31 ± 0.08
Po × 10−2	0.016 ± 0.006	0.022 ± 0.004	1.14*,†† ± 0.24	0.055†† ± 0.023	2.17 ± 0.54	1.61‡ ± 0.07
fo (s−1)	0.41 ± 0.16	0.22 ± 0.07	11.8*,† ± 3.34	0.48 ± 0.12	60.0 ± 41.6	13.4‡ ± 3.91
Nevents	327	316	7471	1140	24143	18416
No. patches	4	7	4	7	4	5

τo₁ and τo₂ represent the mean time constants from exponential functions fitted to the probability density functions of channel open times, a_o1 and a_o2 the magnitude of the contribution to an exponential function, P_o the open probability, f_o the frequency of openings, N_events the total number of events, and No. patches the number of individual patches used for the calculation of the parameters. Mean values ± standard error of mean (SEM) are given. ^*, (^**), the difference in the mean value is statistically significant at the p < 0.05 (0.01) level, in comparisons between PKA-cs- and PP2A-treated patches, at the same internal concentration of G_β/γ. ^†, (^††), the difference in the mean value is statistically significant at the p < 0.05 (0.01) level, in comparisons between recordings obtained at 0 nM and 10 nM G_β/γ. ^‡, the difference in the mean value is statistically significant at the p < 0.05 level, in comparisons between recordings obtained at 10 nM and 100 nM G_β/γ.