TABLE 2.

Single channel closed state parameters in inside-out patches

	Basal		10 nM Gβ1/γ2		100 nM Gβ1/γ2
	PKA-cs	PP2A	PKA-cs	PP2A	PKA-cs	PP2A
τc1 (ms)	0.85 ± 0.22	0.94 ± 0.28	0.44 ± 0.03	0.58 ± 0.14	0.51 ± 0.03	0.52 ± 0.02
τc2 (ms)	n.d.	n.d.	4.37 ± 1.29	5.14 ± 1.25	6.48 ± 0.62	4.24 ± 0.96
τc3 (ms)	n.d.	n.d.	22.1 ± 3.9	54.6 ± 13.9	31.7* ± 3.0	41.9 ± 2.9
τc4 (ms)	n.d.	n.d.	192 ± 27	979 ± 329	179 ± 19	333 ± 107
τc5 (s)	4.47 ± 1.32	11.0 ± 3.2	1.42** ± 0.37	11.2 ± 2.0	3.09 ± 1.27	2.85‡‡ ± 0.50
ac1	0.17* ± 0.07	0.43 ± 0.07	0.33 ± 0.06	0.32 ± 0.07	0.40 ± 0.08	0.47 ± 0.09
ac2	n.d.	n.d.	0.17 ± 0.04	0.17 ± 0.05	0.27 ± 0.03	0.17 ± 0.04
ac3	n.d.	n.d.	0.27* ± 0.05	0.07 ± 0.03	0.23 ± 0.04	0.21 ± 0.06
ac4	n.d.	n.d.	0.16 ± 0.06	0.14 ± 0.04	0.09 ± 0.01	0.12 ± 0.05
ac5	0.83 ± 0.07	0.57 ± 0.07	0.07**;††† ± 0.02	0.30 ± 0.07	0.01 ± 0.00	0.03‡‡ ± 0.01

τc₁–τc₅ represent the mean time constants from exponential functions fitted to the probability density functions of channel closed times and a_c1–a_c5 the magnitude of the contribution to an individual exponential function, respectively. Mean values ± standard error of mean value (SEM) are given. ^*, (^**), the difference in the mean value is statistically significant at the p < 0.05 (0.01) level, in comparisons between PKA-cs- and PP₂A-treated patches, at the same internal concentration of G_β/γ. ^†††, the difference in the mean value is statistically significant at the p < 0.001 level, in comparisons between recordings obtained at 0 nM and 10 nM G_β/γ. ^‡‡, the difference in the mean value is statistically significant at the p < 0.01 level, in comparisons between recordings obtained at 10 nM and 100 nM G_β/γ. n.d., states could not be detected.