FIGURE 7.

(A) Tilt angle θ of the H3 helix with respect to the bilayer normal during room-temperature free-dynamics runs of the wild-type (WT) and the prosequence mutant (N₁₀), both embedded within uncharged (POP⁰) and charged (POP⁻) bilayer. The period that was used for the free-energy landscape estimate, G(θ), is shaded gray. (B) Smoothed free-energy landscape for the H3 helix tilt angle, derived from the four free-dynamics runs of 2-ns length each, according to Eq. 1. (C) Linker helicities determined from the four 2-ns free-dynamics runs. Shown are the relative frequencies p_helix,i that each of the five linker residues is in helical configuration, given that one of its neighbors is helical.