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. 2003 Mar;84(3):1674–1689. doi: 10.1016/S0006-3495(03)74976-5

FIGURE 8.

FIGURE 8

High concentrations of suramin or DBDS do not protect RyRs from fast DIDS activation. (A) Four RyRs in the presence of 1 mM Ca2+ are exposed to 1 mM suramin (solid bar). During this time the RyRs are exposed to 1 μM DIDS for ∼30 s (open bar). In the presence of suramin DIDS further activated the RyRs and activity did not return to control levels after the removal of DIDS and suramin. (B) Effects of DBDS and DIDS on a single RyR shown in consecutive traces. The top three traces demonstrate reversible activation by DBDS alone. Traces 4–8 show that 20 s DIDS exposure in the presence of DBDS causes nonreversible activation. In these experiments channel openings produce upward current jumps from the baseline (dashed line). Approximate scales for mean Po are shown at the right of A.