FIGURE 13.

Exit of a small vesicle through a transient pore. The small vesicle (marked by an arrow) of radius R_cargo ≈ 0.6 μm is initially inside a larger one, of radius R ≈ 16 μm (first frame, t = 0 s). When a pore opens (second frame, t = 0.76 s), the small vesicle starts moving, dragged by the leaking fluid. It passes through the pore (fourth frame, t = 3.93 s) just before the pore reseals (sixth frame, t = 5.24 s). The position of the small vesicle and the pore radius are plotted as a function of time in Fig. 14. The vesicles are labeled using the dye RH237 (Fig. 5; also see Materials and Methods).