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. 2003 Mar;84(3):1784–1795. doi: 10.1016/s0006-3495(03)74986-8

FIGURE 2.

FIGURE 2

pH-dependent leakage. (A), Percentage of leakage over time at pH 5.0 and 5 μM lipid: a and b, POD/POPE/DOPE (10/50/40); c, PEG-DSG/POD/POPE/DOPE (4/10/50/36); d, PEG-DSG/POPE/DOPE (10/50/40); a, c, and d in acetate buffer; b in citrate buffer. (B), Percentage of leakage over time at 5 μM lipid in acetate buffer (pH 4.5, far left trace) and phosphate buffer (pH 4.5, 5.0, 5.5, 6.0, 7.0, and 7.4). (C), Logarithm of lag time (tl in seconds) of POD/POPE/DOPE (10/50/40) vesicles at various pH in acetate (⋄), citrate (♦), glucuronate (□), and phosphate (▪) buffers. Model: Log10(tl) = pH + Log10(ln(Ao/Ac)/k). Observed: Log10(tl) = (0.819 ± 0.067) × pH – (1.881 ± 0.395), r = 0.949, and P < 0.0001.