FIGURE 3.

Temperature-dependencies of spectral parameters of sperm whale apomyoglobin at pH^* 3.0, 20 mM NaCl. (a) Fluorescence emission maximum position (λ_max). (b) molar ellipticity at 222 nm ([Θ]_222nm). (c) IR melting transitions at key wavenumbers: 1633 cm⁻¹ (♦), 1648 cm⁻¹ (^*), 1668 cm⁻¹ (•). The solid curve in a and b is the van't Hoff analysis fit to the E-form data, taking into account a finite heat capacity change between the folded and unfolded structures (see text).