Skip to main content
. 2003 Apr;84(4):2393–2415. doi: 10.1016/S0006-3495(03)75045-0

TABLE 2.

Ratio L2(Å) L3(Å) L2 + L3(Å) θtilt(°)
Vpu2–37/DLgPC
 1:∞ 12.96 12.64 25.60 29.4
 1:50 11.14 12.60 23.74 36.8
 1:40 12.33 11.60 23.90 36.3
 1:20 13.10 11.07 24.17 36.0
 1:15 13.65 10.96 24.61 34.9
 1:10 12.44 11.97 24.41 36.2
Vpu2–51/DLgPC
 1:∞ 13.10 12.37 25.50 29.9
 1:50 13.39 10.52 23.91 36.2
 1:40 13.88 10.07 23.95 36.1
 1:20 14.48 9.88 24.36 35.3
 1:15 13.93 10.72 24.65 34.7
 1:10 13.91 10.94 24.85 34.8
Vpu2–81/DLgPC
 1:∞ 13.10 12.37 25.50 29.9
 1:50 10.80 13.30 24.10 35.5
 1:40 11.86 12.24 24.10 35.6
 1:20 12.04 12.40 24.40 35.2
 1:15 13.38 11.03 24.86 34.0
 1:10 14.07 10.80 24.87 34.7
Average thickness tHCR of the hydrocarbon chain region (HCR), as obtained from the best nonlinear least-squares fits of the sum of four Gaussian functions to the gradients of the monolayer electron density profiles dρ(z)/dz from Box Refinement, for mixed Tm/DLgPC, TmCy/DLgPC, and Vpu/DLgPC monolayers as a function of increasing protein/DLgPC mole ratio at surface pressure of 45 mN/m and T = 20°C. Here, tHCR = L2 + L3 where the L2 and L3 parameters from the best fits are the separation of the second and third interfaces and the separation of the third and fourth interfaces, respectively, bounding the two layers (“slabs”) required to best represent the hydrocarbon core region of the monolayer profiles. The estimated errors in tHCR were found to be ±0.1 Å, based on plots of the integral of the residuals, defined here as
graphic file with name M13.gif
where the subscripts BR and GM refer to the Box Refinement and four-Gaussian Model respectively, as a function of the positions of the second and fourth interfaces.