TABLE 2.
Fiber sample | Maximal tension | pCa50 | n (Hill coefficient) |
---|---|---|---|
Untreated + DTT + TnC | 279.67 ± 13.63 | 6.289 ± 0.071 | 4.670 ± 0.47 |
ES + DTT + TnC | 224.30 ± 26.25 | 6.183 ± 0.004 | 1.601 ± 0.045 |
rRLC + DTT + TnC | 270.24 ± 15.23 | 6.310 ± 0.048 | 3.154 ± 0.021 |
vRLC + DTT + TnC | 275.31 ± 18.51 | 6.515 ± 0.015 | 2.244 ± 0.144 |
The tension is reported as kN/m2. The values are the mean ± SE for four single fibers from two preparations. The difference pCa50 for the untreated + DTT + TnC fibers in Table 2 compared with Table 1 is explained in Materials and Methods. ES + DTT + TnC fibers are rRLC-depleted fibers; rRLC + DTT + TnC are fibers reconstituted with rabbit RLC; and vRLC + DTT + TnC are fibers reconstituted with rat vRLC.