TABLE 1.

Crystal parameters, data collection, and refinement statistics

		Illuminated state
Complex	Dark state LOV1-447	Dataset of a single crystal	Composite dataset of LOV1-390
PDB code	1N9L	1N9N	1N9O
Crystal parameters: P6522 unit cell (a = b, c), Å	122.1, 46.1	120.9, 46.0	121.9, 45.5
Data collection
Beamline (ESRF, Grenoble)	ID14-4	ID29	ID29, ID14-4
Wavelength, Å	0.9393	0.969	0.969/0.939
Data statistics
Resolution, Å	15.0–1.9	15.0–2.3	15.0–2.8
No. of observations/unique reflections	77,830/16142	69,773/9184	16,983/5009
Completeness (total/high) [%]*	98.0/96.6	99.5/99.9	95.2/82.5
I/σ(I) (total/high)*	12.8/3.5	12.6/4.6	7.5/2.6
Rsym,(total/high)*†	6.8/35.1	13.2/43.1	12.9/32.2
Refinement statistics
Resolution range, Å	15.0–1.9	15.0–2.3	15.0–2.8
Included amino acids	17–125	18–125	18–125
No. of protein atoms	844	834	834
No. of waters	108	114	26
No. of SO42− ions	1	0	1
Rwork, Rfree, %‡	23.2/24.8	21.2/26.5	25.0/29.8
Root-mean square deviation for bonds/angles, Å/degrees	0.008/1.28	0.008/1.34	0.009/1.32

^*Completeness, R_sym, and I/σ(I) are given for all data and for the highest resolution shell: dark, 1.9–2.0 Å; illuminated 1, 2.3–2.4 Å; composite dataset, 2.8–2.9 Å.

^†R_sym = ∑|I − 〈I〉|/∑I.

^‡R_work = ∑|F_obs| − k|F_calc|/∑|F_obs|.

5% of randomly chosen reflections were used.