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. 2003 Apr;84(4):2768–2780. doi: 10.1016/S0006-3495(03)75081-4

FIGURE 2.

FIGURE 2

Effect of divalent cations on the fluorescence excitation spectra of PC:PS lipid vesicles containing 0.2 μM di-4-ANEPPS. A 10 mg/mL stock solution of lipid vesicles was diluted 50-fold into a cuvette containing 0.2 μM di-4-ANEPPS, 140 mM NaCl (pH 7.0). The emission wavelength is 610 nm. (A and C) Excitation spectra for a series of PC:PS vesicle suspensions containing various concentration of Mg and Ba, respectively. The spectra were normalized by the area under each curve to the same integrated intensity. (B and D) Normalized difference excitation spectra for the same PC:PS vesicle suspensions as in A and C, respectively. The difference between the spectra obtained without Mg (or Ba) and with various concentration of Mg (or Ba) is displayed.