FIG. 2.

Neutrophil infection and delayed spontaneous neutrophil apoptosis are dose-dependent responses. Neutrophils were incubated overnight with once-washed tachyzoites that were resuspended in fresh medium at various multiplicities of infection, and cytospin preparations were stained with Diff-Quik. At least 300 cells were examined at magnification ×1,000, and the percentages of apoptotic or infected neutrophils were enumerated. Each result is reported as the mean ± standard deviation of triplicate samples and are representative of three donors.