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Kinetics of the expression of Mcl-1 in stimulated neutrophils. Neutrophils were incubated for various times with or without once-washed tachyzoites that were resuspended in fresh medium (1:2 neutrophil-to-parasite ratio). Proteins in whole-cell lysates were separated by SDS-PAGE, and Western blots were incubated with anti-Mcl-1. Each lane represents 5 × 105 neutrophil cell equivalents. The results are representative of two experiments.
