TABLE 3.

Fluorescence intensity decay parameters for nystatin in interaction with 1 mM LUV prepared with different phospholipids (T = 21°C)

Phospholipid	f1	τ1 (ns)	f2	τ2 (ns)	f3	τ3 (ns)	〈τ〉 (ns)
DPPC*	0.06	1.9	0.15	14	0.79	43	36
POPC†	0.20	1.2	0.45	5.2	0.35	9.5	5.9

f_i and τ_i are the fractional fluorescence intensity and lifetime, respectively, of each decay component. 〈τ〉 is the intensity-weighted mean fluorescence lifetime of the antibiotic. The liposomes were prepared in HEPES buffer.

^*The decay components were independent of nystatin concentration between 1.9 and 12.5 μM. f₁, ±0.01; τ₁, ±0.6; f₂, ±0.02; τ₂, ±1.0; f₃, ±0.03; τ₃, ±1.0; 〈τ〉, ±1.0.

^†The fluorescence intensity decays were independent of nystatin concentration between 3.5 and 12.5 μM. They were globally analyzed by linking the lifetimes of each fluorescence intensity decay (n = 7), a of 1.1 being obtained. f₁, ±0.01; f₂, ±0.02; f₃, ±0.04; 〈τ〉, ±0.2.