TABLE 4.

Fluorescence intensity decay parameters for nystatin in interaction with SUV of DPPC as a function of phospholipid and antibiotic concentration (T = 21°C)

[DPPC] (mM)	[Nystatin] (μM)	f1 ± 0.07	τ1 (ns) ± 0.8	f2 ± 0.05	τ2 (ns) ± 1.3	f3 ± 0.05	τ3 (ns) ± 3	〈τ〉 (ns) ± 2
1.1	1.9	0.20	1.4	0.66	7.0	0.14	29	9.0
	3.8	0.24	2.7	0.54	7.9	0.22	28	11
	4.7	0.20	2.3	0.51	8.2	0.29	33	14
	5.4	0.16	2.7	0.30	10	0.54	38	24
	6.3	0.12	2.5	0.27	10	0.61	38	26
	6.7	0.11	2.4	0.24	10	0.65	38	27
	7.9	0.08	1.9	0.23	10	0.69	39	29
	9.4	0.11	1.9	0.20	12	0.69	40	30
	12.5	0.08	2.1	0.20	12	0.72	40	31
3.5	3.2	0.36	3.0	0.60	8.0	0.05	28	7.3
	4.7	0.33	2.6	0.60	8.1	0.07	28	7.7
	6.3	0.29	2.6	0.61	7.9	0.10	27	8.3
	7.9	0.24	2.7	0.56	8.2	0.19	32	11
	9.4	0.24	2.8	0.54	8.3	0.23	33	13
	11.0	0.26	2.9	0.48	8.7	0.25	34	13
	12.5	0.09	2.9	0.29	9.9	0.62	37	26
	14	0.08	2.4	0.22	11	0.70	39	30

f_i and τ_i are the fractional fluorescence intensity and lifetime, respectively, of each decay component. 〈τ〉 is the intensity-weighted mean fluorescence lifetime of the antibiotic. The liposomes were prepared in HEPES buffer.