TABLE 5.
Determination of nystatin aggregation number, z, in gel-phase DPPC vesicles
| z* | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | ||
| K′ag* (μmol)1−z | 9.6 × 10−3 | 3.0 × 10−3 | 1.0 × 10−3 | 3.7 × 10−4 | 1.4 × 10−4 | 5.3 × 10−5 | 2.0 × 10−5 | 8.0 × 10−6 | 3.1 × 10−6 | 1.2 × 10−6 | |
| RMS | 5.36 | 4.70 | 4.48 | 4.44 | 4.48 | 4.56 | 4.65 | 4.74 | 4.83 | 4.91 | |
The cooperative partition model of nystatin in interaction with the lipid vesicles was fitted to the experimental data of 〈τ〉 versus 〈A〉 (n = 86) according to the procedure described in the text. K′ag was allowed to vary in each minimization of the root-mean-square deviations (RMSD), while z was kept constant at different integer numbers. These results are presented in the inset of Fig. 4.
*
Kag can be obtained from Eq. 11.