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Absorption spectra of (a) WT-PYP, (b) L-PYP, and (c) E46Q, in Tris-HCl buffer (pH 7.5) at 298 K (solid line), in glycerol-buffer (55:45 v:v) solution at 298 K (dotted line), glycerol-buffer glass at 77 K of dark-state sample (dot-dash line).
