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Michaelis-Menten kinetics is observed with the substrate methyl-FLIP. (A) Initial rates versus substrate concentration (D-enantiomer). (B) The initial rates are enhanced by the presence of the nonsubstrate lipid, diC6PC (0.69 mM). The solid lines are fits to the Michaelis-Menten equation. Parameters are given in Table 2. The PI-PLC concentration was 12.9 pM.
