TABLE 2.

Kinetic parameters for the methyl-FLIP activity of B. cereus PI-PLC obtained by fitting to the Michaelis-Menten equation

Data set	Curve shape	diC6PC (mM)	(mM)	(s−1)
Fig. 6 A	Hyperbolic	0	0.81 ± 0.01	101 ± 5
Fig. 6 B	Hyperbolic	0.69	0.14 ± 0.01	85 ± 2

Data fit by standard nonlinear regression methods. The uncertainties listed refer to one data set. The variation of between data sets was much greater due to differences in enzyme activity of preparations used on different days (see text). The kinetic constants are apparent values only in the presence of the activator, diC₆PC.