FIGURE 8.

Reduction of endogenous ADP-dependent respiration rate in the presence of 2 mM MgATP by increasing PK activity in the solution in control experiment (A and C) and after trypsin treatment (B and D). Arrows indicate addition of 20 mM Cr into the solution to stimulate respiration. Note the good correlation between simulations and the measurements in control conditions for DF values <0.06. Simulations with different K_m for ATP and ADP of ATPase are compared: A and B correspond to K_m value of 100 μM; and C and D correspond to K_m value of 300 μM. D^app (see Methods and Appendix) in A and C was as in cellular bulk water phase (Aliev and Saks, 1997) reduced by factor DF; and in B and D, as in water solution.