FIGURE 6.

Analysis of NBD-PE and NBD-GD1a bright aggregate cross correlation with membrane structural markers. Cells were dual-labeled with NBD-PE or NBD-GD1a. NBD-PE samples were dual-labeled with (A) WGA-TRX, (B) ConA-biotin, (C) transferrin-biotin, or (D) DiQ. NBD-GD1a samples were dual-labeled with (A) WGA-TRX, (B) ConA-biotin, (C) Transferrin-biotin, or (D) DiQ. Also shown is dual-labeling of ConA-biotin and DiQ (E). Zoom-10 images were collected for each of these samples in two detection channels and the images were modified using ISA to isolate the bright aggregate fluctuations. Shown are the ISA-CD_g (black bar), ISA-CD_r (light gray bar), and ISA-CD_rg (dark gray bar).