FIGURE 2.

Tonic and phasic responses under conventional and perforated whole cell current-clamp conditions. (A) Responses of a tonic neuron to a sequence of depolarizing currents of increasing amplitudes. (B) Responses of a phasic neuron to a similar series of current steps. (C) Both cell types were also observed under the perforated patch-clamp method, using nystatin as the perforating agent (see Methods). (Left) Response of a tonic neuron to a current step. (Right) Response of a phasic neuron to an identical stimulus.