TABLE 2.
Mutational analyses of pVir
| Class of gene | None | Annotation | Insertion point (bp)/orientationa | Relative invasionb |
|---|---|---|---|---|
| Type IV | Cjp1 | virB8 | 72/+ | 89.8 ± 12.6 |
| Cjp2 | virB9 | 722/+ | 29.9 ± 13.9d | |
| Cjp6 | virD4 | 5873/+ | 105.1 ± 12.9 | |
| Cjp53 | virB4 | 35722/+ | 127.4 ± 12.9 | |
| CJ homolog | Cjp32 | Cj0041 | 23163/− | 34.8 ± 8.5d |
| HP homologs | Cjp8 | JHP0926 | 6658f/− | 112.9 ± 10.3 |
| Cjp11 | topA | 9249/+ | 99.2 ± 4.9 | |
| Cjp26 | HP1000/JHP0935 | 16839/− | 84.1 ± 8.3 | |
| Cjp49 | HP0996 | 31996/− | 26.0 ± 8.3c | |
| Known function | Cjp13 | ssb | 12133/+ | 93.0 ± 1.8 |
| Unknowns | Cjp12 | 11407/− | 94.7 ± 4.9 | |
| Cjp15 | 12994/− | 53.6 ± 9.7e | ||
| Cjp16 | 13218/+ | 79.0 ± 12.3 | ||
| Cjp23 | 15765/− | 69.3 ± 9.3 | ||
| Cjp24 | 16067/+ | 88.0 ± 10.3 | ||
| Cjp29 | 20215/+ | 15.2 ± 8.2c | ||
| Cjp30 | 21163/+ | 98.5 ± 17.7 | ||
| Cjp44 | 29123/− | 88.9 ± 12.0 | ||
| Cjp51 | 34533/− | 76.9 ± 1.9 |
a
The insertion point of each transposon insertion was determined by DNA sequence analyses using primers within the transposon (13, 14); a plus sign indicates that the cat gene was inserted in the same orientation as the target gene; a minus sign indicates that the cat gene was inserted on the opposite strand as the target gene.
b
Actual invasion of wild-type 81-176 was 2.43% ± 0.58% of the inoculum. Values are given relative to 81-176, which was set at 100%.
c
P < 0.0001.
d
P < 0.001.
e
P < 0.01.
f
Insertion is in the noncoding region between Cjp7 and Cjp8.