FIG. 1.
Effect of blocking integrins as well as mannose receptors on the uptake of M. tuberculosis grown under anaerobiosis by macrophages cultured in the presence of 10% autologous serum. Laboratory conditions were defined as 37°C, 20% O2, pH 7.2, and isoosmolarity; anaerobiosis was obtained by using an anaerobic jar as described in Materials and Methods. Antibodies were added to monolayers 1 h prior to the experiment at 23°C at concentrations of 30 μg/ml. Anti-P. aeruginosa lipid A monoclonal antibody (IgG) was used at 30 μg/ml as a nonrelevant antibody. No inhibition of uptake was observed. α-Methyl-mannoside was used at a concentration of 1 μg/ml. *, P < 0.05 compared with control.