. 2002 Nov;70(11):6223–6230. doi: 10.1128/IAI.70.11.6223-6230.2002

TABLE 6.

Effect of preopsonization on the induction of TNF-α and IL-12 production by macrophages

Experimental group	Presence of opsonization^a	Production of cytokine (pg/ml)^b
Experimental group	Presence of opsonization^a	TNF-α	IL-12
Laboratory conditions	−	430 ± 40	65 ± 10
	+	505 ± 52	69 ± 4
Anaerobiosis	−	110 ± 14^c	10 ± 2^c
	+	293 ± 26^c	18 ± 3^c
pH 6.0	−	135 ± 37^c	8 ± 1^c
	+	232 ± 20^c	17 ± 5^c

M. tuberculosis was opsonized with 10% serum at 37°C for 30 min and then used to infect macrophages in the presence of 10% heat-inactivated serum.

Cytokine production was measured by ELISA. Results are means × standard deviations. The experiment was repeated three times. Results are means ± standard deviations.

P < 0.05 compared with laboratory conditions.