TABLE 2.

Estimation of bend angle (θ) and persistence length (P)

	AFM		TEB
	θ(°)	P(nm)	θ(°)	P(nm)
471 bp	40–50	60 ± 7	38 ± 7*	—
476 bp	0	62 ± 8	0	44 ± 2†

^*Average bend angles calculated from the ratios of the TEB τ-values of the bent 471-bp fragment and reference τ-values of normal fragments of the same molecular weight in several low ionic strength buffers (Lu et al., 2003).

^†Global P-value estimated from the TEB τ-values using the Hagerman-Zimm/Newman formalism for a set of normal, unbent fragments in a buffer containing 1 mM Tris/0.1 mM EDTA/0.2 mM MgCl₂ (Lu et al., 2002). The individual P-value calculated for the 476-bp fragment was 48 ± 3 nm, slightly higher than the global value.