TABLE 1.

Estimates of secondary structure in PrP (113–127) from analysis of CD spectra

	% of secondary structure for PrP (113–127)
	α-helix	β-sheet	β-turn	Random coil
a. %TFE
0	5.2	44.9	16.5	31.4
5	5.3	44.9	18.6	29.1
10	3.6	45.4	21.6	32.9
15	3.3	40.6	25.5	32.0
20	5.9	36.7	24.9	32.2
25	6.7	34.5	26.8	38.5
30	14.0	26.2	23.1	29.6
40	24.8	23.4	21.6	31.5
50	31.5	18.9	19.5	29.2
60	34.4	16.9	19.3	28.5
70	36.3	11.1	20.0	31.0
80	38.4	9.6	18.3	31.4
90	35.1	11.5	20.4	30.5
95	36.4	12.4	19.7	30.0
100	36.0	15.4	19.1	29.1
b. Concentration, mM
0.17	41.7	14.8	18.7	26.0
0.86	38.0	15.8	19.6	27.0
1.21	33.9	18.7	18.8	28.0
1.73	30.2	21.5	20.1	27.9
2.25	25.0	27.0	21.4	27.4
2.95	24.3	29.9	20.2	26.0
c. 5% SDS solution	44.7	13.6	16.4	26.6
d. pH
2	1.7	43.4	21.2	32.4
5	6.2	43.0	18.7	29.9
7	5.2	44.9	16.5	31.4
10.6	22.5	41.6	14.8	22.0