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ESI-FTICR mass spectra showing the 8+ charge-state of CaM with V11F peptide (concentration ratio 1:1.5) in ammonium acetate buffer, 5 mM, pH 5.9, containing (A) 0.1 mM CaCl2, (B) 0.1 mM CaCl2 and 0.05 mM MgCl2, (C) 0.05 mM CaCl2 and 0.1 mM MgCl2, and (D) 0.01 mM CaCl2 and 0.1 mM MgCl2. C represents CaM and P peptide, respectively.
