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Changes in IL-8 mRNA levels in Hc hepatocytes treated with TNF-α. Total RNA was prepared from Hc cells at the indicated time points after exposure to TNF-α (20 ng/ml). Twenty micrograms of RNA was separated by electrophoresis on 1% agarose gel and transferred to nylon membranes. The membranes were hybridized with the specific probe against IL-8 synthesized by RT-PCR. The results shown are representative of at least two independent experiments.
