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(A) Solid-phase assay for in vitro binding of bovine hemoglobin to whole P. multocida wild-type (lane 1) or hgbA mutant (lane 2) cells. (B) In vivo quantitative analysis of hemoglobin adsorption to P. multocida wild-type (▪) and hgbA (▴) cells. The behavior of E. coli cells (•) was used as a negative control. All values are the means of three experiments (each performed in triplicate), and the standard error of any value was never greater than 10%.
