TABLE 5.

Partition coefficients (Π) of PEGs and cyclodextrins into wild-type and mutated αHL pores

Pore	PEG or CD	Π1	Π2	Πall sum	Πall measured
WT-αHL	PEG-0.94k	0.88 ± 0.12	0.36 ± 0.04	1.2	1.1 ± 0.2
WT-αHL	PEG-2.0k	0.24 ± 0.06	0.13 ± 0.02	0.37	0.29 ± 0.03
WT-αHL	PEG-3.1k	0.032 ± 0.004	0.028 ± 0.005	0.060	0.052 ± 0.006
WT-αHL	PEG-4.2k	0.012 ± 0.003	0.013 ± 0.002	0.025	0.021 ± 0.004
WT-αHL	PEG-4.6k	0.013 ± 0.003	0.0083 ± 0.0021	0.021	0.020 ± 0.003
WT-αHL	PEG-6.0k	0.011 ± 0.003	0.0072 ± 0.0014	0.018	0.017 ± 0.003
WT-αHL	βCD-1.1k	83*		n.a.	n.a.
M113N-αHL	βCD-1.1k	8.3 × 105*		n.a.	n.a.
N139Q-αHL	s7βCD-1.7k†	6.0 × 105‡		n.a.	n.a.

Π₁ and Π₂ are the partition coefficients of the free PEGs into the lumen of the αHL pore for the short and long occupancy events, respectively, as derived from the kinetic analysis. For the cyclodextrins, the overall partition coefficient is given, calculated from K_f values as described in the text. The overall partition coefficient for the PEGs was calculated by using Π_all = Π₁ + Π₂ or measured directly from the uncorrected total times in occupied and unoccupied states.

^*From Gu et al. (1999).

^†s₇βCD is hepta-6-sulfato-βCD.

^‡From Gu et al. (2001a).