TABLE 1.
Multiparameter selection of H. pylori antigens
| Antigen | Genea | Seroreactivityb,c | Staining intensityc,d | Surface localizationc,e | Distribution in 15 isolatesc,f | Predicted T-cell epitopesc,g | Homology to other organismsh | Protection |
|---|---|---|---|---|---|---|---|---|
| HP0010 | groEL | + | 5,596 | Surf. | All genomes | 5 | Strong (1.6e − 193) | Yes (11) |
| HP0011 | groES | + | 1,477 | ND | NA | NA | NA | NA |
| HP0027 | icd | + | 683 | ND | NA | NA | NA | NA |
| HP0072 | ureB | + | 3,544 | Surf. | All genomes | 3 | Strong (6.4e − 158) | Yes (25) |
| HP0073 | ureA | + | 1,774 | Surf. | All genomes | 2 | Strong (3e − 199) | Yes (25) |
| HP0109 | dnaK | + | 177 | Surf. | All genomes | 8 | Strong (4.9e − 57) | NA |
| HP0115 | flaB | + | <100 | ND | NA | NA | NA | NA |
| HP0153 | recA | + | 209 | ND | NA | NA | NA | NA |
| HP0154 | eno | + | 214 | ND | NA | NA | NA | NA |
| HP0175 | cell binding factor 2 gene | + | 655 | Sec., Surf. | All genomes | 3 | Strong (9.3e − 31) | NA |
| HP0177 | efp | + | 340 | ND | NA | NA | NA | NA |
| HP0192 | frdA | + | 264 | ND | NA | NA | NA | NA |
| HP0231 | Hypo. ORF | + | 531 | Sec., Surf. | All genomes | 3 | Weak (1.8e − 06) | Yes (this study) |
| HP0243 | napA | + | 673 | Surf. | All genomes | 1 | Strong (3.5e − 22) | Yes (33) |
| HP0264 | clpB | + | 241 | ND | NA | NA | NA | NA |
| HP0305 | Hypo. ORF | + | 320 | ND | NA | NA | NA | NA |
| HP0318 | Cons. hypo. ORF | + | 227 | ND | NA | NA | NA | NA |
| HP0371 | fabE | + | 340 | ND | NA | NA | NA | NA |
| HP0400 | lytB | + | 130 | ND | NA | NA | NA | NA |
| HP0410 | hpaA homologue | + | 415 | Surf. | All genomes | 4 | None | Yesi (this study) |
| HP0512 | glnA | + | 397 | ND | NA | NA | NA | NA |
| HP0522 | cag3 | + | 93 | ND | NA | NA | NA | NA |
| HP0537 | cag16 | + | 76 | ND | NA | NA | NA | NA |
| HP0547 | cag26 | + | 1,194 | Sec. | 11 of 15 | 6 | Weak (0.00012) | Yes (22) |
| HP0589 | Ferredoxin oxidoreductase/gene | + | 263 | ND | NA | NA | NA | NA |
| HP0599 | hylB | + | 302 | ND | NA | NA | NA | NA |
| HP0601 | flaA | + | 450 | Surf. | All genomes | 3 | Strong (4.7e − 91) | NA |
| HP0649 | aspA | + | 340 | ND | NA | NA | NA | NA |
| HP0691 | yxjD | + | 237 | ND | NA | NA | NA | NA |
| HP0752 | fliD | + | 230 | ND | NA | NA | NA | NA |
| HP0779 | acnB | + | 843 | ND | NA | NA | NA | NA |
| HP0794 | clpP | + | 237 | ND | NA | NA | NA | NA |
| HP0795 | tig | + | 450 | ND | NA | NA | NA | NA |
| HP0829 | quaB | + | 212 | ND | NA | NA | NA | NA |
| HP0875 | Catalase gene | + | 1,021 | Surf. | All genomes | 2 | Strong (9.4e − 157) | Yes (28) |
| HP0900 | hypB | + | 341 | ND | NA | NA | NA | NA |
| HP0912 | omp20 | + | 150 | Surf. | NA | NA | NA | NA |
| HP1018 | Hypo. ORF | + | 150 | ND | NA | NA | NA | NA |
| HP1019 | htrA | + | 603 | Sec., Surf. | All genomes | 4 | Strong (2.3e − 91) | NA |
| HP1037 | pepO | + | 213 | ND | NA | NA | NA | NA |
| HP1098 | Cons. hypo. secreted ORF | + | 646 | Surf. | All genomes | 6 | Strong (1.7e − 36) | NA |
| HP1110 | Pyruvate ferredoxin oxidoreductase gene | + | 250 | ND | NA | NA | NA | NA |
| HP1125 | omp18 | + | 900 | Surf. | All genomes | 1 | Strong (1.3e − 23) | NA |
| HP1132 | atpD | + | 171 | ND | NA | NA | NA | NA |
| HP1134 | atpA | + | 162 | ND | NA | NA | NA | NA |
| HP1152 | ffh | + | 124 | ND | NA | NA | NA | NA |
| HP1199 | rpl7/l12 | + | 915 | ND | NA | NA | NA | NA |
| HP1201 | rlpA | + | 398 | ND | NA | NA | NA | NA |
| HP1205 | tufB | + | 727 | ND | NA | NA | NA | NA |
| HP1285 | Cons. hypo. ORF | + | 59 | ND | NA | NA | NA | NA |
| HP1293 | rpoA | + | 273 | ND | NA | NA | NA | NA |
| HP1302 | recA | + | 603 | ND | NA | NA | NA | NA |
| HP1307 | rplE | + | 397 | ND | NA | NA | NA | NA |
| HP1350 | Protease gene | + | 154 | ND | NA | NA | NA | NA |
| HP1555 | tsf | + | 251 | ND | NA | NA | NA | NA |
| HP1563 | tsaA | + | 2,122 | ND | NA | NA | NA | NA |
| HP1564 | omp | + | 434 | Surf. | All genomes | 3 | Strong (5.4e − 58) | NA |
| HP1582 | pdxJ | + | 355 | ND | NA | NA | NA | NA |
Abbreviations: Hypo., hypothetical; ORF, open reading frame; Cons., conserved.
The particular criteria matching proteins are boxed.
Arbitrary units as determined from spot intensities of H. pylori two-dimensional gels using the TOPSPOT program (16). An arbitrary threshold of 175 equivalent to 0.1% of the total staining intensity was used.
Data from references 1, 5, 15, 26, 27, and 30. Abbreviations: Sec., secreted; Surf., surface; ND, not detected.
Presence as determined by DNA-microarray hybridization (31). NA, not analyzed.
Murine H-2d major histocompatibility complex class II-restricted T-cell epitopes were predicted by comparing the amino acid sequence to those of known T-cell epitopes (20); the number of nonoverlapping epitopes with a predicted score equal or higher than that of the well-defined ovalbumin T-cell epitope (amino acids 323 to 339) are given. NA, not analyzed.
Homology (P value) for the closest non-Helicobacter homologue in the Comprehensive Microbial Database (at http://www.tigr.org). A P value below 1.0 × 10−20 is called strong homology. A P value of 0.001 to 1.0 × 10−20 is called weak homology. NA, not analyzed.
If present in challenge strain.