TABLE 2.

The summary of equilibrium association constants defining the equilibrium between the on and off conformations of scHMM in the presence and absence of calcium

		Value
Conditions	Parameter	+Ca2+	−Ca2+
100 mM KCl	*	15 (94% on)	10−2 (<1% on)
	Keq†	v. large (>99% on)	∼2 (∼67% on)
20 mM KCl	*	2 (67% on)	2 × 10−3 (<0.1% on)
	Keq†	20 (>94% on)	∼2 (∼67% on)

^*Data from the analysis of mant-ADP replacement with ATP experiments.

^†Estimations from the amplitudes measured in mant-ADP binding experiments. K_eq = [on]/[off], in the absence (K_eq) or presence of ADP. The contribution of on heads to the total scHMM population was calculated from K_eq as: on/(on + off) = K_eq/(1 + K_eq).