TABLE 1.

Fluorescence spectral parameters of 2′-deoxyadenosine (dA) in aqueous mixtures of methanol, isopropanol, n-propanol, and isobutanol with water contents of 8% and 15%: ratio f of the fluorescence peak intensity for the aqueous mixture to that for the neat organic solvent, wavelength of the fluorescence spectral peak λ_p (in nm), and full width at half maximum (FWHM) (in cm⁻¹) as defined in Eq. 1

	0% water			8% water			15% water
Solvent	f	λp (nm)	FWHM (cm−1)	f	λp (nm)	FWHM (cm−1)	f	λp (nm)	FWHM (cm−1)
Methanol	1	315	4244	1.1	313	4102	1.2	314	4076
Isopropanol	1	320	4301	1.5	320	4705	0.7	320	3764
n-propanol	1	322	4853	1.1	327	4882	1.6	323	4794
Isobutanol	1	318	4125	1.0	325	4833	1.0*	317*	3696*

For dA in water, λ_p = 319 ± 2 nm and FWHM = 4570 ± 270 cm⁻¹. The errors are: ∼±10% for f, ∼±2 nm for λ_p, and ∼±6% for FWHM.

^*For isobutanol, the maximum water content used was 12% by volume due to the low solubility of water in isobutanol, 14% (Marcus, 1998).