TABLE 2.
Fluorescence spectral parameters of 2′-deoxyguanosine (dG) in aqueous mixtures of methanol, isopropanol, n-propanol, and isobutanol with water contents of 8% and 15%: ratio f of the fluorescence peak intensity for the aqueous mixture to that for the neat organic solvent, wavelength of the fluorescence spectral peak λp (in nm), and full width at two-third maximum FWTTM (in cm−1)
| 0% water
|
8% water
|
15% water
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Solvent | f | λp (nm) | FWTTM (cm−1) | f | λp (nm) | FWTTM (cm−1) | f | λp (nm) | FWTTM (cm−1) |
| Methanol | 1 | 329 | 4290 | 1.1 | 330 | 4226 | 1.3 | 332 | 4277 |
| Isopropanol | 1 | 333 | 4945 | 1.5 | 328 | 4212 | 1.3 | 332 | 4366 |
| n-propanol | 1 | 324 | 4689 | 1.3 | 334 | 4325 | 1.2 | 336 | 6791 |
| Isobutanol | 1 | 326 | 3961 | 1.0 | 327 | 3857 | 1.4* | 326* | 3840* |
For dG in water, λp = 327 ± 2 nm and FWTTM = 5040 ± 260 cm−1.
*
For isobutanol, the maximum water content used was 12% by volume due to the low solubility of water in isobutanol, 14% (Marcus, 1998).