FIGURE 7.

Distinguishing kinetochore microtubules from non-kinetochore microtubules, based on differences in flux. The two histograms represent the velocity distribution of the complete speckle population moving upward (solid line) and of speckles located below the MP only (dashed line), respectively. The second population therefore contains only non-kinetochore microtubules. The two velocity distributions are very similar, but the population containing only non-kinetochore microtubules exhibits slightly higher velocities, making the distributions significantly different, as judged by a Kolmogorov-Smirnov statistical test (p-value = 0.0004).