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. 2002 Nov;70(11):6215–6222. doi: 10.1128/IAI.70.11.6215-6222.2002

FIG. 4.

FIG. 4.

Antibodies induced in GalT KO mice by oral inoculation are specific for [Gal α-1,3-Gal]. Sera (1:5) were absorbed with 20 mM synthetic [Gal α-1,3-Gal] disaccharide for 18 h at 4°C. Duplicate samples were incubated with 20 mM lactose [Gal β-1,4-Glc] as a negative disaccharide control. Sera were then assayed for antibody binding to GalT+ target cells as described in the text. (A) Control mouse sera. The open histogram indicates results for the C57BL/6 serum. The filled histogram indicates results for the naive GalT KO serum. Target cells incubated only with secondary antibody yielded identical binding results. (B) Immunized GalT KO mice. The open histogram indicates results for the C57BL/6 serum control. The filled histogram indicates results for the serum from inoculated GalT KO mice. (C) Serum from inoculated GalT KO mice absorbed with 20 mM synthetic [Gal α-1,3-Gal] disaccharide (filled histogram) or 20 mM lactose (open histogram). Sera from nine representative immunized mice were assayed with similar results.