FIG. 2.
The protective effect of αGalCer is CD1d dependent. (A) BALB/c CD1d−/− or CD1d+/+ mice were inoculated intravenously with 4 × 106, 1 × 106, or 0.25 × 106 CFU of M. tuberculosis. Each group contained five to eight mice, and there were no statistically significant differences in survival of the CD1d−/− mice compared to that of the CD1d+/+ mice. (B) BALB/c CD1d−/− and CD1d+/+ mice were inoculated intravenously with M. tuberculosis (3 × 106 CFU/mouse) and subsequently treated with αGalCer or vehicle on days 1, 5, and 9 following infection. Only BALB/c CD1d+/+ mice treated with αGalCer survived longer than the vehicle-treated mice (P < 0.0001). Arrows, administration of αGalCer or vehicle. WT, wild type.