TABLE 1.
Parameter values of the model
| Pancreatic cells | |||
| kf (apical) | 0.71 s−1 | kf (basal) | 0.32 s−1 |
| v1 (apical) | 0.098 s−1 | v1 (basal) | 0.04 s−1 |
| v1 (buffer region) | 0.08 s−1 | Vmito (buffer region) | 100 s−1 |
| Parotid cells | |||
| kf (apical) | 5.9 s−1 | kf (basal) | 1.2 s−1 |
| v1 (apical) | 0.24 s−1 | v1 (basal) | 0 s−1 |
| v1 (buffer region) | 0.08 s−1 | Vmito (buffer region) | 100 s−1 |
| Transport parameters | |||
| Jer | 0.002 s−1 | γ | 5.405 |
| Vserca | 120 (μM)2s−1 | Kserca | 0.18 μM |
| Vpm | 28 μM s−1 | Kpm | 0.425 μM |
| δ | 0.1 | Dc | 20 (μm)2 s−1 |
| IPR parameters | |||
| k1 | 0.64 s−1 (μM)−1 | k−1 | 0.04 s−1 |
| k2 | 37.4 s−1 (μM)−1 | k−2 | 1.4 s−1 |
| k3 | 0.11 s−1 (μM)−1 | k−3 | 29.8 s−1 |
| k4 | 4.0 s−1 (μM)−1 | k−4 | 0.54 s−1 |
| L1 | 0.12 μM | l2 | 1.7 s−1 |
| L3 | 0.025 μM | l4 | 1.7 (μM)−1 s−1 |
| L5 | 54.7 μM | l6 | 4707 s−1 |
| RyR parameters | |||
|
1500 (μM)4 s−1 |  |
28.8 s−1 |
|
1500 (μM)3s−1 |  |
385.9 s−1 |
|
1.75 s−1 |  |
0.1 s−1 |
The IPR parameters were determined by fitting to model data from type-II hepatocyte IPR (Sneyd and Dufour, 2002). The RyR parameters were determined by fitting to model data from cardiac cells (Keizer and Levine, 1996). The transport parameters came from a variety of previous modeling work, as discussed in the text. The remaining parameters were determined by fitting to experimental data from pancreatic and parotid cells (Fig. 4).