TABLE 1.
KCNE4 mRNA expression in mouse tissues and human T-cells.
| Tissue | CP(KCNE4) (Mean ± SD) | CP(GADPH) (Mean ± SD) | CP(KCNE4) – CP(GADPH) | Normalized (KCNE4) (relative to uterus) |
|---|---|---|---|---|
| Brain | 28.6 ± 0.4 | 20.2 ± 0.7 | 8.4 ± 0.9 | 0.01 |
| Cerebellum | 31.8 ± 0.5 | 20.9 ± 1.1 | 10.9 ± 1.2 | 0.01 |
| Hippocampus | 32.9 ± 0.5 | 21.3 ± 0.9 | 11.6 ± 1.0 | 0.0004 |
| Cortex | 32.4 ± 0.8 | 21.1 ± 0.8 | 11.2 ± 1.1 | 0.0006 |
| Hypothalamus | 31.2 ± 0.5 | 20.8 ± 0.8 | 10.4 ± 0.9 | 0.001 |
| Medulla spinalis | 31.9 ± 0.4 | 20.8 ± 0.9 | 11.1 ± 1.0 | 0.0007 |
| Uterus | 26.3 ± 0.3 | 23.5 ± 1.0 | 2.8 ± 1.1 | 1 |
| Kidney | 26.8 ± 0.1 | 20.9 ± 0.8 | 5.9 ± 0.8 | 0.11 |
| Lung | 29.6 ± 0.3 | 25.0 ± 1.1 | 4.7 ± 1.1 | 0.30 |
| Colon | 28.4 ± 0.09 | 22.8 ± 0.8 | 5.6 ± 0.8 | 0.15 |
| Small intestine | 28.0 ± 0.3 | 22.4 ± 1.1 | 5.6 ± 1.1 | 0.15 |
| Heart | 28.3 ± 0.08 | 19.8 ± 0.9 | 8.5 ± 0.9 | 0.009 |
| Embryo | 26.9 ± 0.4 | 22.7 ± 0.9 | 4.3 ± 1.0 | 0.60 |
| CHO-K1 | >45 | 32.0 ± 1.5 | >13 | <0.0001 |
| T-lymphocytes | ||||
| Unstimulated | 30.6 ± 0.3 | 21.2 ± 0.2 | 9.4 ± 0.4 | nd |
| Stimulated | 30.5 ± 0.3 | 21.9 ± 0.6 | 8.6 ± 0.6 | nd |
Real-time RT-PCR analyses of total RNA extracted from NMRI mice tissue, 14-day-old-embryo Swiss Webster mice (Ambion), CHO-K1 cells, and human T-lymphocytes. CP (crossing point) is the PCR-cycle number where a 0.01 fluorescent signal is detected (Opticon Monitor™ software). nd, not done.