TABLE 1.
Primer sequencea | Restriction site | Target gene |
---|---|---|
5′-CCGGATCCCCGTATGAAGCGAAGCTAGC-3′ | BamHI | sspA |
5′-TCAGAATTCATAGTCAGTTTTAGC-3′ | EcoRI | sspA |
5′-CCGGATCCCCGTATGAGGCTAAGCTAGC-3′ | BamHI | sspB |
5′-TCAGAATTCATAATCTGTTTTAGC-3′ | EcoRI | sspB |
5′-CCGGATCCCCGTATGAAGCTAAATTGGC-3′ | BamHI | spaP |
5′-TCAGAATTCGTAATCAGCTTTAGC-3′ | EcoRI | spaP |
Restriction sites are indicated in boldface.